multi well plates  (Bio-Rad)

Journal: Biomedicines

Article Title: Alzheimer’s Disease-Associated Molecular Abnormalities in White Matter Glia and Related Pathologies Detected in Unfractionated and O4-Selected Serum Exosomes Using a Liquid Biopsy Approach

doi: 10.3390/biomedicines14010251

Figure Lengend Snippet: AD effects on immature and non-myelinating oligodendrocyte glycoprotein mRNA expression in frontal lobe white matter. Control (n = 8) and AD (n = 8) mRNA transcripts were measured by qRT-PCR using a Roche LightCycler 480 System. Violin plots (mean, quartiles, and range) display results corresponding to ( A ) CNPase , ( B ) GALC , ( C ) PLP1 , and ( D ) PDGFR-α. Relative mRNA abundance was calculated using the 2 −ΔΔCt method with results normalized to internal control genes. Data were analyzed by two-way ANOVA with post hoc Tukey multiple-comparison tests. Significant ( p ≤ 0.05) differences are shown within the panels. See for the full gene names, PCR primer sequences, and gene functions.

Article Snippet: Targeted arrays were constructed by spotting and drying primer pairs (10 pmol/10 μL) into individual wells of a Lightcycler 480 Multi-well Plate 96 (Roche, Indianapolis, IN, USA).

Techniques: Expressing, Control, Quantitative RT-PCR, Comparison

Journal: Biomedicines

Article Title: Alzheimer’s Disease-Associated Molecular Abnormalities in White Matter Glia and Related Pathologies Detected in Unfractionated and O4-Selected Serum Exosomes Using a Liquid Biopsy Approach

doi: 10.3390/biomedicines14010251

Figure Lengend Snippet: AD effects on mature oligodendrocyte/myelin glycoprotein mRNA expression in frontal lobe white matter. Control (n = 8) and AD (n = 8) purified RNA was reverse-transcribed to generate cDNA, and gene expression was measured by qRT-PCR using a Roche LightCycler 480 System. Violin plots (mean, quartiles, and range) display results corresponding to ( A ) MOG , ( B ) MAG , and ( C ) MBP. Relative mRNA abundance was calculated using the 2 −ΔΔCt method with results normalized to internal control genes. Data were first analyzed by two-way ANOVA followed by post hoc Tukey multiple-comparison tests. The corresponding significant ( p ≤ 0.05) differences are shown within the panels. See for the full gene names, PCR primer sequences, and gene functions.

Article Snippet: Targeted arrays were constructed by spotting and drying primer pairs (10 pmol/10 μL) into individual wells of a Lightcycler 480 Multi-well Plate 96 (Roche, Indianapolis, IN, USA).

Techniques: Expressing, Control, Purification, Reverse Transcription, Gene Expression, Quantitative RT-PCR, Comparison

Journal: Biomedicines

Article Title: Alzheimer’s Disease-Associated Molecular Abnormalities in White Matter Glia and Related Pathologies Detected in Unfractionated and O4-Selected Serum Exosomes Using a Liquid Biopsy Approach

doi: 10.3390/biomedicines14010251

Figure Lengend Snippet: AD effects on expression of astrocytic mRNA transcripts in frontal lobe white matter. Control (n = 8) and AD (n = 8) purified RNA was reverse-transcribed to generate cDNA, and gene expression was measured by qRT-PCR using a Roche LightCycler 480 System. Violin plots (mean, quartiles, and range) display results corresponding to ( A ) Nestin , ( B ) Vimentin , and ( C ) GFAP. Relative mRNA abundance was calculated using the 2 −ΔΔCt method with results normalized to internal control genes. Data were first analyzed by two-way ANOVA followed by post hoc Tukey multiple-comparison tests. The corresponding significant ( p ≤ 0.05) differences are shown within the panels. See for the full gene names, PCR primer sequences, and gene functions.

Article Snippet: Targeted arrays were constructed by spotting and drying primer pairs (10 pmol/10 μL) into individual wells of a Lightcycler 480 Multi-well Plate 96 (Roche, Indianapolis, IN, USA).

Techniques: Expressing, Control, Purification, Reverse Transcription, Gene Expression, Quantitative RT-PCR, Comparison